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Complex manufacturing processes and the need for precise control over particle properties hinder scalability, while immune responses and inefficient BBB targeting present additional obstacles

The freshly obtained lung tissues were processed into small rectangular pieces and were rinsed with advanced DMEM/F12 medium (Gibco) supplemented with 2 mM of HEPES (Gibco), 1 GlutaMAX (Gibco), 100 U ml 1 penicillin and 100 g ml 1 streptomycin

Proc Natl Acad Sci U S A (2015) 112(50):1540813.10.1073/pnas.1512832112 41 MorawskiPABollandS

Kidney organoids: accurate models or fortunate accidents

Eggs, dietary choline, and nonalcoholic fatty liver disease in the framingham heart study