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The cells were incubated in blocking solution (3% BSA, 5% milk, 50% FBS, 0.1% Triton X-100, and 0.05% NaN 3 in PBS) for 2 h, washed and incubated in the presence of anti-LC3 antibodies in PBS solution (containing 3% BSA, 0.1% Triton X-100, and 0.05% NaN 3 ) overnight at 4 C

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[36] [37] Since antiarrhythmic drugs may be ineffective or may be arrhythmogenic in patients with hypokalemia, any potassium or magnesium deficiency should be corrected before and during amiodarone therapy