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Three primary forms of autophagy, macroautophagy, microautophagy, and molecular chaperone-mediated autophagy, are mediated by lysosomes, which are important organelles for the breakdown of proteins (Mizushima and Levine, 2020)

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Firstly, CD206 was employed as the M2 macrophage marker to confirm macrophage polarization status using immunofluorescence staining performed under inflammatory stimuli (LPS and IFN-)