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In addition, to investigate whether intravenous injection of EVs would achieve the therapeutic effect identical to intramyocardial injection, mice were injected with macrophage-derived EVs via the tail vein immediately after LAD ligation

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5.0 mL of the release medium was collected at the set time, and replenished with PBS with the same pH and volume

To discriminate between the role for GSH in either resisting ROS or as a nutrient source, we sought to determine if depleting GSH increased ROS

Note: Note: When the absorbance is greater than 1.2, it is suggested that the sample be determined after diluted with the extraction It is recommended not to take too many samples at a time, to avoid the influence of too long testing time on color development, which may let the determination is not Experimental instances Experimental instances Take 0.1g of mouse liver, add 1mL of extract solution, homogenate, and grind