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Recent studies demonstrate the presence of apical mitochondria, which may contribute to maintaining the RPE-photoreceptor connection and cooperation [86, 90, 91]

Equal amounts of proteins from lysates were separated by SDSPAGE using Mini-PROTEAN TGX 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad)

Although specialized FBS formulations remain valuable for certain niche applications, the overall trend favours GMP-compliant, XF, and ethically sourced media to improve reproducibility and translational relevance