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For non-radioactive assays, 200 M cold ATP was used and phosphorylation was analysed by immunoblotting with phospho-specific antibodies

ISBN 978-1-4160-2973-1

Journal of Investigative Dermatology (2001)

Recent studies indicate that the gut microbiota and its metabolites are crucial for neurodevelopment, neurodegenerative diseases, and other neurological disorders (McCallum and Tropini, 2024)

However, gaps remain in the understanding of subset functions and regulatory mechanisms: in vitro studies rely on limited cell lines (e.g., HD11 macrophages, DF-1 fibroblasts), and the lack of long-term primary immune cell culture systems hinders simulation of in vivo cellular functions in complex microenvironments