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Microbiol Res 281:127602

Thomas C, and So BT

PubMed Kolik, L.G., Nadorova, A.V., & Kozlovskaya, M.M

Sci Rep 11 , 6985 (2021)

Overall, this scalable pH shift strategy, particularly when using Bis-Tris as a pre-elution buffer and amino acids in the elution buffer, offers a viable approach for mitigating harsh low pH elution conditions, thereby enhancing protein stability and yield