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Fasting in the context of CR is required for the benefits on insulin sensitivity, frailty, cognition, and lifespan in wild-type mice 12,54 , and indeed fasting alone without reduction of calories is sufficient for many of the molecular effects of CR as well as extending lifespan in wild-type mice 12,55

These risks highlight the need for individualized assessments before intervention, standardized dosing and duration, and long-term safety monitoring to ensure clinical applicability and minimize potential harms

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Signs of dehydration include dark urine, dizziness, and increased thirst