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The resulting retention of NaCl in the TALH increases the osmolarity of the fluid remaining in the lumen and therefore facilitates greater water retention via osmosis at a later stage of the nephron [180]

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The advantage of Lumio staining is that one can do both in vivo and in vitro protein labeling

Calibration data for the bioluminescence assay is provided in Additional file 1: Figures S12 and S13