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For glutathione and glutamate quantification, cell viability analysis and oxidative stress assay, cells were sub-cultured in 12-well plates at a density of 4.2 10 5 cells/well

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While direct causal evidence in PCOS remains limited, this framework integrates endocrine, metabolic, and immunological dimensions to provide a structured lens for understanding how neutrophil and NET-mediated pathways could contribute to systemic and organ-specific dysfunction in affected women

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