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Thus, it is essential to apply standardized experimental conditions, including oxygen concentration and administration, duration, and cellular context, to ensure reproducibility among studies that facilitates meaningful comparisons and minimizes conflicting interpretations [99]

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If the solution becomes crystal-clear with zero residual haze, it may be safe to use

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Based on mechanistic studies of the bystander effects, the underlying mechanisms can be classified into two categories: (i) the existence of physical contact between irradiated and non-irradiated cells, which generates intercellular communication mainly through gap junctions (31), and (ii) the release of a series of soluble signaling molecules by irradiated cells that mediate the bystander effect in non-irradiated cells (30)