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Affinity Chromatography Using Protein A Sepharose and Protein G Sepharose The basis for purification of IgG, IgG fragments and subclasses is the high affinity of protein A (cell wall protein derived from Staphylococcus aureus ) and protein G( derived from groups C and G Streptococci )for the Fc region of polyclonal and monoclonal IgG-type antibodies

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This reduction aligns with previous research by Harvie et al