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For IL-1 treatment, cells were incubated in plain Dulbeccos modified Eagles medium (DMEM):F12 medium containing 6 mM glucose and 2 mM glutamine overnight followed by stimulation with IL-1 (10 ng/ml) or 0.1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) (vehicle control)

Consistent with our observations, CYP9Q2 and CYP9Q3 are known to be constitutively expressed at low levels in bees 17

Hepatic veins of monkeys with veno-occlusive disease

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and improved selective attention during mental tasks likely due to changes in alpha brain wave activity (25)