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TRPM2 channel aggravates CNS inflammation and cognitive impairment via activation of microglia in chronic cerebral hypoperfusion

MTT solution was added to each well, followed by incubation for 1 h at a final concentration of 5 mg/mL in 5% CO 2 at 37 C

Sorias laboratory, originally generated by Vogelstein laboratory

A major obstacle to effective treatment is the inability of many neuroprotective agents to reach the brain at therapeutically relevant concentrations due to poor bioavailability and the restrictive nature of the bloodbrain barrier

These nutraceuticals have some mechanisms of action in common, including the inhibition of ACE, the enhancement of NO-mediated vasodilation, interactions with ion channels including the blocking of calcium channels and opening of potassium channels with consequent membrane hyperpolarization, anti-inflammatory effects with reductions in synthesis and release of pro-inflammatory cytokines, and enhancement of endogenous antioxidant actions exerted by different enzymes, such as SOD, GPx, and GSTP