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If you're drinking more and more quickly than your body can keep up, your glutathione levels plummet, leaving behind an army of free radicals and leftover acetaldehyde

10.1124/jpet.122.001194 19 HayA.DziewulskaK.GamboniF.NerguizianD.DzieciatkowskaM.ZimringJ

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After culturing for another 24 h, the cells were washed three times with PBS and then stained in 100 L serum-free DMEM supplemented with DCFH-DA (10 M) and Hoechst (1 g mL 1 ) at 37 C for 1 h