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For protein digestion, 0.5 g of trypsin (Promega) was added to the samples, followed by an overnight incubation at 37 C

For example, exposure to pyocyanin mediated ROS to human airway epithelial cell activate NF-B and other inflammatory signaling pathways, leading to the production of pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-) and interleukins (IL-1, IL-6, IL-8) (93)