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One or two days after seeding, cell medium was replaced with Hank's Balanced Salt Solution (HBSS) , and keratinocytes were pre-treated with GAP (or 0.12 -0.240 mM sodium hydroxide vehicle control, pH 7.5) alone or in combination with additional test reagents described above or L-cystine esters alone for 1-2 hours

SODs require nutrients copper, zinc, and manganese for form and function, and genes encoding for SODs can dictate much about their function

The concentrations of glutathione were then calculated from slopes of the absorbance changes in reference to the standard curve

As collagen diminishes, wrinkles and sagging appearcopper peptides rebuild the underlying structure, strengthening the skin