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Brain organotypic slice (350 m-thick) cultures were prepared from hippocampi of P4P7 rats through a McIllwain tissue chopper and then placed on semiporous membranes (Millipore) for 57 days before experiments 29

The buoyancy and rate of inflow in the lower section of the device are designed to cause a gradual separation between the lower section and the lid as the device sinks slowly into the water, providing enough time for the experimenter to vacate the testing area

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As a secondary calcium reservoir within cardiomyocytes, mitochondria can buffer Ca 2+ in response to fluctuations in cytosolic calcium levels