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After this period, the cells were washed and sonicated in saline solution, centrifugated to obtain a clean solution, and measured in COBAS C311 autoanalyzer (Roche Diagnostics, IN, USA)

The progression of oxidative stress and change in the functioning of Grx disturb the regulation of S-glutathionylation, which may facilitate a number of pathophysiological changes observed in diabetes, disorders of the lungs and heart, oncological diseases, and different neurodegenerative processes

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For example, activation of CaMKK2 in mice increases the expression of brain-derived neurotrophic factor (BDNF), a pivotal regulator of neuronal function [45]

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