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The lv-shRNAs targeting NF1 were synthesized according to the sequences of si-NF1-1 and si-NF2-1 and inserted into the GV493 vector

Biochemical tools for characterizing individual fungal proteins and genetically manipulating fungal strains have significantly advanced our understanding of enzyme function and central carbon metabolism (i.e., glycolysis/gluconeogenesis, pentose phosphate pathway or PPP, TCA cycle, and transhydrogenase cycle)

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Reported timeline from the published record Window 0-6 days post single dose What was measured GH elevated 2-10 fold (Teichman 2006)

S4 e and f, HEK293T cells were cotransfected with a DNA plasmid encoding Cv::TurboID::KDEL and hCES2 using JetOptimus (Polyplus-transfection) transfection reagent