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Coimmunoprecipitation and Western blotting For coimmunoprecipitation assays, cells were washed twice with PBS and lysed with an NP-40 buffer (100 mM NaCl, 10 mM TrisHCl, pH 7.5, 0.2% NP-40, 10% glycerol) supplemented with cOmplete protease inhibitor cocktail (MilliporeSigma, Roche)

GLP-1, glucagon-like peptide-1

Third, due to the variability and limitations of data from public databases, extensive in vitro and in vivo experiments are still required to support our findings

Typical bag volumes include 10 mL (tubing flushes), 50100 mL (minibags for pediatric or TB inserts), 250 mL, 500 mL, and 1000 mL (1 L)

Apply an ice pack for 1020 minutes after the injection