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Chemistry and pharmacology of analgesic indole alkaloids from the rubiaceous plant, Mitragyna speciosa

Following two washes with Perm/Wash and PBS/BSA solution, cells were resuspended in 80 l PBS/BSA and analyzed on an LSR-II flow cytometer with FACSDiva software (BD Biosciences)

Next, biosensors were dipped into wells containing the second antibody, in the presence of the first competing antibody (all at 100 nM), and binding was measured after 900 s of association