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The supernatant was removed and incubated with anti-Flag M2 agarose beads (Sigma, USA) or with recombinant protein G sepharose beads (Saier, China) at 4 C overnight

The potential inclusion of belzutifan and other HIF-2 inhibitors into the mRCC treatment armamentarium either as a single agent or as combination therapy would be a welcome addition for patients with mRCC