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Similarly, the elevated percentage of CD133 + cells, and protein levels of CD133, EpCAM, NANOG, OCT4, and SOX2 by CRIP1 overexpression were attenuated by iCRT3 (Fig 6D and E), indicating that the Wnt/catenin pathway is involved in CRIP1induced cancer stemlike properties in HCC

Bacterial cell death was measured through incubation with propidium iodide (Pi) which cannot be taken up by viable bacteria

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These observations suggest the importance of correlating various functional properties of infecting spirochetal strains with clinical manifestations in larger-scale translational studies, likely at the site of disease (e.g