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Gel band quantification was performed using Image J, and the apparent dissociation constant (K d ) was calculated by fitting binding curves in GraphPad Prism

IC, Interstitial cystitis

Then, the cartridge was dried under vacuum with a defined nitrogen flow (5.0, Nippon Gases, Dsseldorf, Germany) for approximately 25 min

Given that ARID1A-negative cancer cells have been previously shown to be more susceptible to inhibition of glutathione synthesis compared to ARID1A-positive cancer cells, we next sought to determine whether EN25 selectively impairs cell viability of ARID1A-negative A2780 ovarian cancer cells, compared to ARID1A-positive RMG-1 ovarian cancer cells

Nevertheless, further studies are required to assess the clinical relevance of this inflammatory process in the onset and progression of COPD [69]