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Ferroptosis-related detection Mitochondrial membrane potential detection Mitochondrial membrane potential was measured by fluorescence microscopy of cells using Mitochondrial Membrane Potential Assay Kit with TMRE (Beyotime, China)

7), consistent with the observed half-maximal degradation concentration (DC 50 ) values (MV4-11 1000 mm 3 within 12 days (Supplementary Fig

This feedback mechanism suggests the peptide may help prevent excessive tissue growth while still promoting repair

Second, translational development should focus on dual-functional nanocarriers incorporating A-targeting peptides and RVG29 brain-homing peptides, delivering GPX4 activators or iron chelators with pH/ROS-responsive release to ensure lesion-specific delivery and avoid neurotransmitter synthesis impairment from non-specific iron depletion