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Metabolites and lipids were examined in two independent cohorts of subjects who had lumbar puncture either without exercise (nonexercise group) or after the second of two bouts of submaximal exercise performed on two consecutive days (postexercise) 22,23,24,25

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Other amino acids may enter the cycle as succinyl-CoA, fumarate or pyruvate

All remaining supernatant was removed and 40 L PBS was used to resuspend the pellet