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Description

Pellets were grinded with pestles in 100 l of the same buffer, cells were lysed by two freeze-thaw cycles and insoluble material was removed by filtration in a COSTAR R Spin-X centrifuge tube filter

Subsequently, Na+/Cl+ ion pairs were systematically added to attain a physiological NaCl concentration, emulating intracellular ionic strength conditions 54

And neither study proved that HRT directly caused or prevented tinnitus

All statistical analyses were conducted using GraphPad Prism 8 software

Other parameters used for the SEQUEST searches included the fixed modification of carbamidomethylation at cysteine (+57.021 Da), the variable modification of oxidation at methionine (+15.995 Da)