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For this purpose, 4-chloro-3-hydroxybutyronitrile is synthesized from epichlorohydrin in an aqueous hydrogen cyanide solution, 4-chloro-3-hydroxy butyric acid alkyl ester is then synthesized from the 4-chloro-3-hydroxybutyronitrile in the presence of an alcohol and a sulfuric acid catalyst, and the racemic 4-chloro-3 -hydroxy butyric acid alkyl ester is hydrolyzed in an aqueous solution using an enzyme, thereby preparing (R)-4-chloro-3-hydroxy butyric acid alkyl ester

Oxid Med Cell Longev 2016;2016:2398573

Its unique profile makes it an attractive compound for exploring the therapy of metabolic disease and muscle aging, as well as evaluating the intricate links between metabolism, epigenetics, and tissue function 1-4

They discovered that the clustered repeats had a series of spacer-type sequences, later called CRISPR