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It can be assumed that increased demand in tissue results in a decrease in its plasma concentration

After homogenizing the tissues in a cold buffer (using 20% homogenate), they were centrifuged at 4000 rpm for 15 min at 4 C

When transferrin and TFRC heighten intracellular iron levels, the accompanying surge in free iron can accelerate the Fenton reaction, producing ROS species that damage membranes and drive cells toward ferroptosis ( 2.1.1.2 Solute carrier family 40 member 1 (SLC40A1) SLC40A1, also known as ferroportin or FPN1, serves as the sole transmembrane exporter of non-heme iron in mammalian cells

Treatment can reverse both conditions

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