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Thus, we do not expect that our model will make perfect quantitative predictions

Then, 5 l of Annexin VFITC and 5 l of propidium iodide (PI) were added, and the cells were gently vortexed

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The strong decrease of fluorescence is then caused by the immediate formation of roGFP2 WT (S 2 ) which cannot be kinetically separated from the slower glutathionylation of the roGFP2 moiety (Fig