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Despite these promising works, the exact role of glutathione in AD requires further studies

doi: 10.1038/s41433-020-01378-w 558 SonH

Notably, formation of SnCE1-SUMO1-PA crystals were observed the fastest, appearing within a week compared to apo SnCE1 crystals which took weeks to months to grow

Specifically, it decarboxylates G6P and forms ribose-5-phosphate (R5P) via three irreversible reactions

The harvested cells were resuspended in buffer A (20 mM Tris-HCl, pH 8.0, 300 mM NaCl and 1 mM DTT) for GST-tagged proteins or in buffer B (20 mM Tris-HCl, pH 8.0, 300 mM NaCl, 5% glycerol and 10 mM imidazole) for His-tagged ShHTL7, and they were lysed by sonication