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The mixture was incubated on a rotator at 4 C for 1 h, followed by centrifugation at 15,000 g at 4 C for 10 min

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Intracellular cysteine is taken up primarily in the form of cystine via system Xc 144 or converted from methionine via the transsulfuration pathway, 145 or is transported directly by alanine/serine/cysteine transporters (known as system ASC)

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