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For mtDNA copy number assessment, qPCR has remained the mainstream approach given its low cost and throughput, though accuracy is affected by amplification efficiency and standard curve variability [102, 173, 174]

The anesthesia was assessed by monitoring the loss of pedal reflexes

All treatments were given concurrently for a 30-day period

In laboratory settings, this 5mg format allows researchers to observe the initial coordination of intracellular signaling cascades and biomarker response dynamics