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The immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the I NEXTFLEX ChIP-Seq Library Prep Kit for Illumina Sequencing (NOVA-514120, Bioo Scientific) and sequenced on Illumina X Ten with the 150PE method by Wuhan IGENEBOOK Biotechnology Co., Ltd ( Data analysis The clean reads were then mapped to the reference genome by BSseeker software 49

NAC (N-acetylcysteine) is a precursor, it helps your body make more glutathione, the bodys master antioxidant

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