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Description

We suggest starting with a 1,000 mcg dose given weekly

Denuded oocytes were fixed in 4% PFA for 30 min, followed by incubation in PBS containing 3 mg/ml BSA and 7.5 mg/ml glycine for 15 min

Hsiao et al., 2013)

The concentrations of each group were the same as before

A general rule regarding buffers is that the pH of the buffer solution should be within 1.0 pH unit of the pK a to provide appropriate buffering capacity