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Serum and gastric levels of the pro-inflammatory cytokines TNF- (BT-Lab E0764Ra), IL- (BT-Lab E0119Ra) and IL-10 (BT-Lab E0108Ra) were measured by using rat ELISA kits according to the manufacturer's procedure

Then the tissue sections were transferred onto Millicell cell culture inserts (0.4 M, 30 mm diameter) and placed in 6-well plates containing 1.5 mL of medium consisting of DMEM/F12 with N-2 Supplement and 1% antimycotic/antibiotic