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In two studies of inositol [39, 40], that is, one of eicosapentaenoic acid [41] and one of silymarin [42], a crossover design was used

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FIGURE 1 Proteomic Analysis of Tomato Developing Pollen Grains Following Exposure of the Plants to Heat-Stress Conditions, With and Without Prior Treatment With an Ethylene Releaser Developing pollen grains were harvested from flowers at developmental stages of 4 and 3 days before flower opening (A-4 and A-3, respectively, exhibiting HS-sensitivity and responsiveness to ethephon pre-treatment) derived from Micro-Tom tomato plants, grown at day/night temperatures of 26/22 2 o C, that were exposed to short-term HS conditions (50 o C for 2 h) following treatment with either water or ethephon

These individuals represented an extremely small minority, but highlight the drawbacks of subjective reporting

doi: 10.1007/s00253-004-1851-8 39 RossiGRuggieroPPeppoloniSPancottoLFortunaDLaurettiLet al