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The N-terminus and His-13 are crucial for Cu 2+ binding, and His-6 and His-14 are also implicated

The analyses of 4MSOB ITC and its conjugates were conducted using an Agilent HP1260 Series instrument coupled to an API5000 tandem mass spectrometer (AB Sciex, Darmstadt, Germany) by loading extracted samples onto an Agilent Zorbax Eclipse XDB-C18 column (50 4.6 mm 1.8 m, Agilent Technologies, Waldbronn, Germany) with mobile phase A (0.05% formic acid in milliQ water) and mobile phase B (acetonitrile)

The initial study showed that DS-8273a might be used to eliminate myeloid-derived suppressor cells in advanced cancer patients, but no objective response was seen (Dominguez et al., 2017)

Sci Rep 16 , 5974 (2026)

In the comparison between LPS and Control, cellular senescence and p53 signaling pathways, which are related to ferroptosis, were enriched in the top 20 of KEGG