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High resolution HPLC-MS/MS was performed on a rapid separation liquid chromatography system (Dionex, UltiMate3000, UHPLC) coupled with an ESI-Q-TOF mass spectrometer (Bruker Daltonics, Impact HD)

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muciniphila , decline pathogenic bacteria, such as Aspergillus and Trematode spirochetes , promote the production of intestinal microbial metabolites such as SCFAs, and restore the breakdown and assimilation of glycolysis, amino acid metabolism, and carbohydrates (Liao et al., 2020), rectifying the imbalance in the rat intestinal flora ratio caused by a HFD, reducing the proportion of Firmicutes to Bacteroidetes , safeguarding the intestinal mucosal barrier, improving intestinal permeability, and preventing hyperlipidemia (Zhang et al., 2012)

In Alzheimer's disease patients, MPO peripheral blood concentration was significantly higher in these patients compared to control [139]