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02 Wipe the stoppers Use one alcohol swab on the bacteriostatic water vial stopper and another on the Semax vial stopper

Hydration affects mitochondrial ATP production, which directly impacts fat oxidation capacity

At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader

Start slow and build up to 30 minutes a day of vigorous aerobic exercise