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In: Albert DM, Miller JW, Azar DT, Young LH, editors

Immunofluorescent staining Tissue cryo-sections were subjected to immunolabeling after permeabilization with 1% Triton-X (Sigma-Aldrich) for 5 min and blocking with 2% BSA for 30 min at room temperature (RT)

Lynn B, Veronika H, Melanie G, Joseph L, Luana G, Henry K et al

In a published study, oral administration of 350 mg/kg acetaminophen to pregnant rats (0.85 times the MHDD, based on a body surface area comparison) from Gestation Day 13 to 21 (dams) reduced the number of germ cells in the fetal ovary, decreased ovary weight, and reduced the number of pups per litter in F1 females as well as reduced ovary weights in F2 females