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Usually, the first sign of an iron deficiency is brittle nails that chip and crack easily

There are two ways to measure the B12 supply: directly measure B12 in the blood measure certain decomposition substances in the urine, which accumulate in the instance of deficiency According to both routes there are different methods

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So what are you saying, are you indicating B12 does have adverse effects

The process is called lyophilization, or freeze-drying