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Mutagenesis was conducted using a standard PCR-based overlap extension method with mutation-specific primers, followed by DpnI digestion to remove the methylated parental plasmid

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10.1164/arrd.1977.115.3.479 44 GuX.ChengQ.HeP.ZhangY.JiangZ.ZengY

Archived from the original on 11 January 2010

Donate References Auger J, Jouannet P, Eustache F

By molecular simulation, we have previously shown that YMU1 acts on the catalytic pocket of TMPK, which causes the expulsion of the lid domain to give an open conformation that inhibits the catalytic process 19