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Notably, adipose-specific, but not liver-specific, GPR146 deletion reduces hepatic lipid accumulation by limiting free fatty acid (FFA) influx

In brief, enzyme conjugate (200 l) was added to 25 l of sample and standard (0, 0.2, 0.5, 1, 2, 6, and 16 ng/ml) well, mixed and incubated at room temperature (RT) for one hour