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doi: 10.1016/j.cden.2022.11.003 4

Slides were fixed with 4% paraformaldehyde in PBS for 10 min, washed three times in PBS, permeabilized using a 1:1,000 dilution of Triton X-100 in casein solution, washed another three times in PBS and then blocked for 30 min with casein solution (Thermo Fisher)

Rare but serious reactions include chest pain, irregular heartbeat, or paradoxical bronchospasm (worsening wheeze), all of which require immediate medical attention