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Briefly, RAW 264.7 cells were washed three times with phosphate buffer saline (PBS, Corning) and then incubated in ice-cold hypotonic lysing buffer (50 mM TrisHCl, 10 mM magnesium sulfate at pH 7.5) for 4 h at 4 C

STORAGE Lyophilized 28 C, 12+ months Reconstituted 28 C, 14 days PEER-REVIEWED EVIDENCE Tomas FM, Knowles SE, Owens PC, et al