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[89] [90] Blocking of the NMDA receptor results in analgesia by preventing central sensitization in dorsal horn neurons

doi: 10.22207/JPAM 66 El-Sayed AhmedM

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Experiment Condiitons Cell Line: HeLa cells Pretreatment: DMEM (cystine-free, serum-free), 37, 5 min Uptake conditions0.5 mmol/l sulfasalazine or 2 mol/l erastin / Cystine Analog / DMEM (cystine-free, serum-free), 37, 30 min Instrument: Fluorescent Plate Reader Filter: Ex=485 nm, Em=535 nm Application Data 2: Comparison with Radio Isotope Method Cystine uptake assay, which used to require RI measurement, can now be easily measured with a plate reader

Structure-Function Correlation Use of predictive modeling tools to visualize SSG-modified sites in the context of protein domains or enzymatic cores