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Another group thats at-risk for low B12 levels are those who have had gastric bypass surgery or have other stomach issues, such as ulcers

How Zinc-Binding Systems, Expressed by Human Pathogens, Acquire Zinc from the Colonized Host Environment: A Critical Review on Zincophores

For example, changing Asp222 in AAT to Ala leads to a 10 5 -fold decrease in activity.[69] The difference between this result from mutagenesis and that with deazaPLP is presumably due to the fact that the D222A simply leads to deprotonation of the pyridine nitrogen, whereas deazaPLP fully exchanges the nitrogen for the less electronegative carbon atom