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The sections were then labelled with either BMAL1 (1:200), CLOCK (1:200) or NRF2 (1:100) antibodies diluted in blocking solution, followed by the goat anti-rabbit Alexa Fluor 488 (1:200) secondary antibody for 2 hours

It is also important to evaluate if the psychiatric conditions of the patient are satisfactorily stable with the current medication, for at least several months prior to the surgery and to confirm the ability to provide consent for the surgical procedure (18, 19)